stromal

The Miracle of Birth Converting Placental-derived Adult Stem Cells to Therapeutics Pluristem Therapeutic Inc. (NasdaqCM: PSTI) is a bio-therapeutics company dedicated to the commercialization of allogeneic (non-personalized) cell therapy products derived from the human placenta, an organ that has traditionally been considered medical waste after childbirth. The company uses its PLX products for the treatment of a variety of ischemic and autoimmune disorders where current therapies are unavailable or inadequate. Pluristem's adherent stromal cells are and a non-embryonic, non-controversial source of cells.

LASIK, The Flap Never Heals This results in a weak, central and paracentral hypocellular primitive stromal scar that averages 2.4% as strong as normal comeal stroma. In contrast, the more superficial, flap margin stromal LASIK wound, which is adjacent to the surface epithelium, was found to heal by producing a 10-fold stronger, hypercellular fibrotic stromal scar that reaches maximum tensile strength by approximately 3.5 years after surgery, averaging 28.1% of normal.

愛妥糖~~治~~子宮內膜異位症？為什麼？ 愛妥糖治子宮內膜異位症？為什麼？ PPAR-gamma ligand activation decreases p450 aromatase gene expression in human endometriotic epithelial and stromal cells in vitro CIG treatment significantly decreased p450aromatase mRNA expression in a cell-specific manner in human endometriotic epithelial and stromal cells. Ongoing studies will unravel the underlying molecular and cellular interactions via in vivo animal models and human cells. If our subsequent in vivo animal and human studies show that PPAR-gamma activation facilitates regression of existing endometriotic lesions, thiazolidinediones such as pioglitazone may prove to be clinically useful for endometriosis-related pain and/or infertility. Article Outline • Copyright OBJECTIVE: Endometriotic lesions express increased aromatase (CYP19, p450arom) levels, leading to local production of estrogen and, ultimately, persistence and self-propagation of these lesions. PPAR-gamma ligands have been shown to decrease p450arom in human granulosa cells and endometrial cells. We sought to determine if a PPAR-gamma ligand, ciglitazone (CIG), could affect aromatase expression in endometriotic epithelial and stromal cells. DESIGN: In vitro study using the immortalized human endometriotic epithelial cell line 12Z and stromal cell line 22B. MATERIALS AND METHODS: Endometriotic cells were cultured and subsequently treated with CIG x 24 hours. Treatment groups (n=3 for each experiment) consisted of control (no CIG compared to CIG at 20 μM ...

PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) From the Springer article: PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) by: Kraemer, Bjoern F.; Borst, Oliver; Gehring, Eva-Maria; Schoenberger, Tanja; Urban, Benjamin; Ninci, Elena; Seizer, Peter; Schmidt, Christine;...

GIST, gastrointestinal stromal tumor ジスト、 腫瘍径 imatinib medicaldirect.jp medicaldirect.blog94.fc2.com

Huge GIST Small Bowel Tumor A 50 years old male presented with abdominal distension and vague abdominal pain. Was found to have a huge GIST tumor at about 50 cm from the DJ junction, firmly attached to the bowel wall, but no obstruction. The tumor was resected with segmental resection of the adjacent small bowel and primary anastomosis. The tumor was ~ 30x30 cm and 6 kg. The patient had a smooth recovery.

Gastrointestinal Stromal Tumors (GIST): A rare, life-threatening cancer Gastrointestinal Stromal Tumors (GIST) are a rare, life-threatening form of cancer. Listen to four patient stories about living with the disease. © 2011 Novartis AG

Homing of Stem Cells to Target Tissue

What is a GIST Tumor A gastrointestinal stromal tumor (GIST) is a common mesenchymal tumor of the gastrointestinal tract, making up 1 to 3 percent of all gastrointestinal malignancies. GIST tumors are caused by mutations in the Kit gene or PDGFRA gene. Ajay Maker, MD, surgical oncologist at Advocate Illinois Masonic Medical Center, talks about a recent young woman he treated for the condition.

Mouse GEN cells overexpressing Csx/Nkx2.5 and GATA4 behave like transient amplifying cells This study was published in Exp Cell Res. 313:698-706, 2007. The tilte of this study is "Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells" by Yamada Y, Sakurada K, Takeda Y, Gojo S, Umezawa A. This study was held at BioFrontier Laboratories, Kyowa Hakko Kogyo Co. Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan. Abstract of this study is as below: Bone marrow-derived stromal cells can give rise to cardiomyocytes as well as adipocytes, osteocytes, and chondrocytes in vitro. The existence of mesenchymal stem cells has been proposed, but it remains unclear if a single-cell-derived stem cell stochastically commits toward a cardiac lineage. By single-cell marking, we performed a follow-up study of individual cells during the differentiation of 9-15c mesenchymal stromal cells derived from bone marrow cells. Three types of cells, ie, cardiac myoblasts, cardiac progenitors and multipotent stem cells were differentiated from a single cell, implying that cardiomyocytes are generated stochastically from a single-cell-derived stem cell. We also demonstrated that overexpression of Csx/Nkx2.5 and GATA4, precardiac mesodermal transcription factors, enhanced cardiomyogenic differentiation of 9-15c cells, and the frequency of cardiomyogenic differentiation was increased by co-culturing with fetal cardiomyocytes. Single-cell-derived mesenchymal stem cells ...

PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) From the Springer article: PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) by: Kraemer, Bjoern F.; Borst, Oliver; Gehring, Eva-Maria; Schoenberger, Tanja; Urban, Benjamin; Ninci, Elena; Seizer, Peter; Schmidt, Christine; Bigalke, Boris; Koch, Miriam; Martinovic, Ivo; Daub, Karin; Merz, Tobias; Schwanitz, Laura; Stellos, Konstantinos; Fiesel, Fabienne; Schaller, Martin; Lang, Florian; Gawaz, Meinrad; Lindemann, Stephan; (recording time: 85 min, 6 frames/minute; replay 20 frames/second) Platelet migration on a fibrinogen matrix. (Representative movie out of three experiments) (AVI 5968 kb) Journal: Journal of Molecular Medicine Vol. 88 Issue 12 DOI: 10.1007/s00109-010-0680-8 Published: 2010-11-15

Preparation of 2-dGuo-Treate Thymus Organ Cultures Abstract: In the thymus, immature CD4+8+ thymocytes expressing randomly rearranged T-cell receptor α- and β-chain genes undergo positive and negative selection events based on their ability to recognize self-peptide/major histocompatibility complex (MHC) molecules expressed by thymic stromal cells. In vivo ***ysis of the role of thymic stromal cells during intrathymic selection is made difficult by the cellular complexity of the thymic microenvironment in the steady-state adult thymus, and by the lack of appropriate targeting strategies to manipulate gene expression in particular thymic stromal compartments. We have shown that the thymic microenvironment can be readily manipulated in vitro through the use of reaggregate thymus organ cultures, which allow the preparation of three-dimensional thymus lobes from defined stromal and lymphoid cells. Although other in vitro systems support some aspects of T-cell development, reaggregate thymus organ culture remains the only in vitro system able to support efficient MHC class I and II-mediated thymocyte selection events, and so can be used as an effective tool to study the cellular and molecular regulation of positive and negative selection in the thymus.

957_0145tomy.MOV It has been a long life saving journey since my wife found out she had Endometrial Stromal sarcoma. Thank god we have her today. Healthy and vibrant.

Cardiac Events with Gleevec (Feb. 2007) Novartis is notifying healthcare professionals about new precautions for Gleevec (imatinib mesylate). Gleevec is used to treat certain patients with chronic myeloid leukemia and gastrointestinal stromal tumors. A recent article published in Nature Medicine reported on ten patients taking Gleevec who developed severe congestive heart failure and left ventricular dysfunction. Before starting treatment, most of these patients had pre-existing conditions such as hypertension, diabetes and coronary artery disease. The article also reported on preclinical studies showing that mice treated with the drug developed left ventricular contractile dysfunction. Novartis has now added a precaution to the drug labeling about severe congestive heart failure and left ventricular dysfunction. The company also says that patients with cardiac disease or risk factors for cardiac failure should be monitored carefully, and any patient with signs or symptoms consistent with cardiac failure should be evaluated and treated. Additional Information: FDA MedWatch Safety Alert - Gleevec (imatinib mesylate) -- October 19, 2006. www.fda.gov

Gastrointestinal Stromal Tumor Metastatic to Liver: Role of Interventional Radiology Dr. Sanjay Gupta discusses the role of interventional radiology in the treatment of GIST that has metastasized to the liver

EBCC 7: Ways to predict efficacy of anthracyclines Dr Christine Desmedt discusses research that attempts to predict the efficacy of anthracyclines. The results of this study validate the use of topoisomerase II alpha as a predictive marker for the efficacy of anthracyclines. Further to this, the gene expression profiles in tumour biopsies were correlated against the response to treatment seen in the patients. This research revealed that some gene expression signatures linked with tumour stroma and immune response are associated with the response to anthracyclines. These immune response and stromal signatures were integrated with the TOP2A signature to give an anthracycline based chemo sensitivity score. This score can be used to identify which patients do not need anthracyclines. Dr Christine Desmedt of the Jules Bordet Institute in Brussels, speaking at the 7th European Breast Cancer Conference in Barcelona.

Adult Stem Cell Results & Embryonic Stem Cell Ethics Video Adult Stem Cell Research: Making a Difference Today from Rosenberg Communications, Inc. The Gerard Health Foundation has produced a powerful video on adult stem cell research, showing its advantages over embryonic stem cell research. The public domain video offers useful, yet often ignored, information about adult stem cell research. We believe the information you'll see in the video is essential to making an informed judgment about the debate over this research. This film is consistent with the teachings of the Roman Catholic Church, which state that the ablation of the inner cell mass (ICM) of the blastocyst, which critically and irremediably damages the human embryo, curtailing its development, is a gravely immoral act and consequently is gravely illicit. No end believed to be good, such as the use of stem cells for the preparation of other differentiated cells to be used in what look to be promising therapeutic procedures, can justify an intervention of this kind. A good end does not make right an action which in itself is wrong. An adult stem cell is an undifferentiated cell found among differentiated cells in a tissue or organ, can renew itself, and can differentiate to yield the major specialized cell types of the tissue or organ. The primary roles of adult stem cells in a living organism are to maintain and repair the tissue in which they are found. Some scientists now use the term somatic stem cell instead of adult stem cell. Unlike embryonic stem cells, which ...

Cardiomyogeneisis of murine marrow stromal cells Bone marrow derived stromal cells can give rise to cardiomyocytes as well as adipocytes, osteocytes, and chondrocytes in vitro. The existence of mesenchymal stem cells has been proposed, but it remains unclear if a single-cell-derived stem cell stochastically commits toward a cardiac lineage. By single-cell marking, we performed a follow-up study of individual cells during the differentiation of 9-15c mesenchymal stromal cells derived from bone marrow cells. Three types of cells, ie, cardiac myoblasts, cardiac progenitors and multipotent stem cells were differentiated from a single cell, implying that cardiomyocytes are generated stochastically from a single cell-derived stem cell.

Project FLAG: Families Learning About GIST - Dana-Farber Cancer Institute In this video, Dr. George Demetri of Dana-Farber's Center for Sarcoma and Bone Oncology discusses gastrointestinal stromal tumor (GIST) and describes the aims of Project FLAG, a National Institutes of Health-funded study to learn more about a rare type of hereditary GIST. Learn more about Project FLAG: www.dana-

Gastrointestinal Stromal Tumor (GIST) News from the 2011 ASCO Annual Meeting Dr. Mark Kris discusses the news highlighted at ASCO's 2011 Annual Meeting that covers research on managing cancer as a chronic disease, and includes information on advances in treatment for gastrointestinal stromal tumors (GIST). For more information, visit ().

Stomach Tumor (GIST) Removal Video showing the removal of a stomach tumor (Gastrointestinal Stromal Tumor or GIST). This was a subtotal gastrectomy which means that a significant portion -- up to 75% - of the stomach was removed. Visit our website at .

Last gift to my Mother This is a video of my family and I at Seaworld 2007. My mother had always wanted to go so I saved up every penny I had for months plus quite a bit of my college financial aid to pay for everyone to go. I wanted to do something for everyone to spend time together as a family for her because she was sick but deserved it too. So this video is for my family. And the beat is done by my wife. :) Sorry, the video quality is bad, and this is all the footage I had. UPDATE 2009: My mother had a gastrointestinal stromal tumor (GIST). Essentially that is a tumor in the intestine. Well, it was discovered a few years back, and the doctors said that it was benign and that they should have surgery to take it out. Because my family was one of millions that have no insurance (because of the corrupt system we have) and we couldnt afford it they decided not to get the surgery. We found out a year later that it was indeed malignant and it had spread to her stomach. After 4 more years of a painful deterioration my mother passed away Aug 8, 2008. She was 38 years old... I hope this in some way incourages people to support healthcare reform, it is needed. My mother did not need to die at 38 years old. (anyway, I thought i should update so that everyones questions could be answered.)

Enrollment and Participation in Project FLAG - Dana-Farber Cancer Institute In this video, Dr. Suzanne George of Dana-Farber's Center for Sarcoma and Bone Oncology explains how patients with GIST can learn more about enrollment and participation in Project FLAG, a National Institutes of Health-funded study to learn more about hereditary gastrointestinal stromal tumor. Learn more about Project FLAG: www.dana-

Mesenchymal stem cells and marrow stromal cells---2nd--- Marrow stromal cells The existence of nonhematopoietic cells in bone marrow was first suggested by Cohnheim about 130 years ago [16]. To date, marrow stromal cells have been shown to differentiate into osteoblasts [17], chondrocytes [18], skeletal myocytes, adipocytes, and cardiomyocytes [19] in vitro, and thus are a useful cell source for regenerative medicine [20, 21]. Recent studies suggest that bone marrow stromal cells can also differentiate into a neuronal lineage [14], and murine bone marrow derived multipotent adult progenitor cell differentiate into dopaminergic neuronal cells [22]. Since the use of bone marrow stromal cells entails no ethical or immunological problems, and bone marrow aspiration is an established routine procedure, they may be a useful source of cells for transplantation.

Anterior stromal puncture 48 hours later The thin outer layer of my cornea was removed on Wednesday, January 14, as part of it had ripped. I have anterior basement membrane dystrophy (ABMD), which means the epithelium doesn't stick onto my cornea properly, and has got so bad I now suffer with recurrant corneal erosions (RCE). The flapping erosion was cut off, and all the loose epithelium. Then I had anterior stromal puncture, basically more than 100 little holes poked into the surface of the cornea, to encourage the epithelium to grow back and stick in place. It didn't hurt at the time because it looked like I was watching pins being pushed into geletine. That geletine was my eyeball. An hour later it hurt. Two hours later it hurt like hell. Imagine a thin layer of skin on your arm being removed and hundreds of little pin pricks pushed into that area. It would feel sore and painful, that's what happened to my eye. This video was filmed at about noon on Friday, January 16, 2008, when I was feeling much better.

Deep Lamellar Keratoplasty - Big bubble technique www.al- This video demonstrates a lamellar keratoplasty technique to bare Descemet's membrane in which air is injected to detach the central Descemet's. After a partial-thickness corneal trephination is performed, a disposable needle is inserted, deeply and bevel down, into the paracentral corneal stroma and air is injected. In most cases, this forms a large air bubble between Descemet's membrane and the corneal stroma. After anterior lamellar keratectomy is performed, a small opening is made in the air bubble and the remaining stromal layers are excised with scissors. This technique is faster, safer, and easier to perform than previous methods

Reaggregate Thymus Cultures Abstract: In the thymus, immature CD4+8+ thymocytes expressing randomly rearranged T-cell receptor α- and β-chain genes undergo positive and negative selection events based on their ability to recognize self-peptide/major histocompatibility complex (MHC) molecules expressed by thymic stromal cells. In vivo ***ysis of the role of thymic stromal cells during intrathymic selection is made difficult by the cellular complexity of the thymic microenvironment in the steady-state adult thymus, and by the lack of appropriate targeting strategies to manipulate gene expression in particular thymic stromal compartments. We have shown that the thymic microenvironment can be readily manipulated in vitro through the use of reaggregate thymus organ cultures, which allow the preparation of three-dimensional thymus lobes from defined stromal and lymphoid cells. Although other in vitro systems support some aspects of T-cell development, reaggregate thymus organ culture remains the only in vitro system able to support efficient MHC class I and II-mediated thymocyte selection events, and so can be used as an effective tool to study the cellular and molecular regulation of positive and negative selection in the thymus.

Prostate cancer simulation Computer simulation of prostate cancer initiation and progression. In this example after a brief period of time the three prostatic glands show cancer initiation from the luminal epithelial cells. After a long period in which the tumours remain in situ (PIN), one of them manages to break up after degrading the basal membrane and despite all the efforts of the stromal cells. Code: red cells=cancer, green and blue = epithelial, black and brown = stroma. From Basanta et al. Cancer Res. 2009. m.nih.gov

PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) From the Springer article: PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) by: Kraemer, Bjoern F.; Borst, Oliver; Gehring, Eva-Maria; Schoenberger, Tanja; Urban, Benjamin; Ninci, Elena; Seizer, Peter; Schmidt, Christine; Bigalke, Boris; Koch, Miriam; Martinovic, Ivo; Daub, Karin; Merz, Tobias; Schwanitz, Laura; Stellos, Konstantinos; Fiesel, Fabienne; Schaller, Martin; Lang, Florian; Gawaz, Meinrad; Lindemann, Stephan; (recording time: 180 min, 30 frames/hour; replay 10 frames/second) Platelet chemotaxis toward a source of SDF-1. (Representative movie out of three experiments) (AVI 4567 kb) Journal: Journal of Molecular Medicine Vol. 88 Issue 12 DOI: 10.1007/s00109-010-0680-8 Published: 2010-11-15

ASCO 2011: Improved Treatment for Gastrointestinal Stromal Tumors - City of Hope's Joseph Kim, MD Joseph Kim, MD, Associate Professor of General Oncologic Surgery at City of Hope, attended the 2011 Annual Meeting of the American Society of Clinical Oncology (ASCO), the world's largest and preeminent cancer meeting. A highlight of the conference for him was the presentation of a study that is a major step forward for patients with gastrointestinal stromal tumors. Learn more about City of Hope research at

PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) From the Springer article: PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) by: Kraemer, Bjoern F.; Borst, Oliver; Gehring, Eva-Maria; Schoenberger, Tanja; Urban, Benjamin; Ninci, Elena; Seizer, Peter; Schmidt, Christine; Bigalke, Boris; Koch, Miriam; Martinovic, Ivo; Daub, Karin; Merz, Tobias; Schwanitz, Laura; Stellos, Konstantinos; Fiesel, Fabienne; Schaller, Martin; Lang, Florian; Gawaz, Meinrad; Lindemann, Stephan; (recording time: 57 min, six frames/minute; replay 20 frames/second) Example of a platelet that migrates toward the source of SDF-1 in the platelet cohort shown in Movie I. SDF-1 source at the top of the image (AVI 1091 kb) Journal: Journal of Molecular Medicine Vol. 88 Issue 12 DOI: 10.1007/s00109-010-0680-8 Published: 2010-11-15

Mesenchymal stem cells and marrow stromal cells Marrow stromal cells are able to generate a series of terminally differentiated cells in vitro. However, most of these experiments are performed with heterogeneous stromal cells, which had been obtained by adherence to plastic culture dishes. Since it is demonstrated that bone marrow-derived stromal cells are purified to a homogeneous population that met the criteria for nonhematopoietic stem cells, these cells have been termed mesenchymal stem cells because they generate an array of cells, defined as mesenchymal cells. In contrast, mesenchymal stem cells are multipotent cells capable of differentiating into cells of mesoderm-origin regardless of cell sources. Mesenchymal stem cells can be recovered from a variety of other adult tissues such as fat, muscle, menstrual blood, endometrium, placenta, umbilical cord, cord blood, skin, and eye. The terms mesenchymal stem cell and stromal cell, both of which are most plausible cells for regenerative medicine, have been used interchangeably in emerging literature; we here re-organize our understanding on two MSC biology and further clinical trail in this review.

IntraLase IntraLASIK iLASIK bladeless all-laser LASIK The latest LASIK industry hype is all-laser LASIK, which involves cutting the flap with a femtosecond laser instead of a blade. Eliminating the use of a blade on your eye sounds like a good thing intially, but wait until you read about the problems, risks, and complications of this technology. The LASIK industry markets this technology uder a variety of names and descriptions. Here are a few: IntraLase, IntraLASIK, femtosecond LASIK, blade-free LASIK, all-laser LASIK, bladeless LASIK, and iLASIK when combined with VISX custom LASIK. Some of the reported complications of femtosecond lasers are opaque bubble layer, corrugated corneal stromal bed, extreme light sensitivity, partial flaps, rainbow glare, torn flap, vertical gas breakthrough, epithelial ingrowth, flap necrosis, diffuse lamellar keratitis (DLK), corneal perforation, incomplete flap, decentered flaps, small flaps, slipped flaps, flap folds, traumatic flap dislocation, gas bubbles in the anterior chamber, optic nerve damage, flap tears, haze, macular hemorrhage, infection, stromal cell necrosis, inflammation, keratocyte cell death, corneal nerve damage, goblet cell reduction, and interface particles. Read more about the side effects, risks, complications, and problems of bladeless LASIK at

PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) From the Springer article: PI3 kinase-dependent stimulation of platelet migration by stromal cell-derived factor 1 (SDF-1) by: Kraemer, Bjoern F.; Borst, Oliver; Gehring, Eva-Maria; Schoenberger, Tanja; Urban, Benjamin; Ninci, Elena; Seizer, Peter; Schmidt, Christine; Bigalke, Boris; Koch, Miriam; Martinovic, Ivo; Daub, Karin; Merz, Tobias; Schwanitz, Laura; Stellos, Konstantinos; Fiesel, Fabienne; Schaller, Martin; Lang, Florian; Gawaz, Meinrad; Lindemann, Stephan; (recording time: 20 min, six frames/minute; replay 20 frames/second) Inhibition of platelet migration with cytochalasin B. (Representative movie out of three experiments) (AVI 2188 kb) Journal: Journal of Molecular Medicine Vol. 88 Issue 12 DOI: 10.1007/s00109-010-0680-8 Published: 2010-11-15

laparoscopic wide excision of GIST gastrointestinal stromal tumor lesser curve stomach

Gastrointestinal stromal tumour

CS4 - Confocal Microscope Nidek introduces the ConfoScan 4, a Confocal Digital Microscope. While a Specular Microscope can only see the endothelial layer through a clear cornea, NIDEK's confocal microscope images all the layers of the cornea automatically. The Confoscan4 is a scanning slit microscope that allows one to see all the corneal layers in vivo. At 25 frames per second, the Confoscan4 images quickly, even through poor media. This delivers a series of images, like an optical biopsy, that includes the Superficial Epithelium, Intermediate Epithelial Cells, Basal Epithelial Cells, Nerve Plexus, Stroma, and Endothelium. For cornea specialists, the ConfoScan4 follows a wide variety of pathologies, allowing for specific treatment to begin immediately. This includes Keratoconus, Stromal Rejection, Guttae, Sands of the Sahara, Fuchs'endothelial dystrophy, Activated KC, and Haze. This can also show Fungal Hyphae and Acanthamoeba without having to take painful cultures from the patient, allowing for treatment to begin immediately. Here is an example of an acute Alkali Burn after eight days, with the undamaged endothelium only visible with the ConfoScan 4. And an example of tear film microscopy. For more information about the ConfoScan4, please contact Nidek today.

Laparoscopic Partial Gastrectomy for Gastrointestinal Stromal Tumors Gastrointestinal stromal tumors are rare lesions stemming from connective tissue anywhere along the length of the gut. This video reviews treatment of such a tumor through Laparoscopic Partial Gastrectomy performed by Mark Liberman, MD For more information, visit .

Resezione laparoscopica di Gastro Intestinal Stromal Tumor

N5-FG-108-all_30fps.AVI Fluorescent time lapse of GFP+ bone marrow stem cells on irradiated primary stroma. Approx 2 months elapsed time. Three stromal cultures are shown - topleft, topright, and bottom right. Bottom left is same as topleft, except it has not had the fluorescence corrected by processing out background autofluorescence using a red fluorescence image subtraction. Top left stroma were seeded with CD34-LKS+ sorted cells (LKS+ = Lineage negative, c-Kit +, Sca-1+). Top right seeded with CD34+LKS+, and Bottom right seeded with LKS-. Fluorescent images were acquired every 100 minutes. Visible images acquired every 10 min (not shown). Magnification is 20x and each panel (4 panels in all) consists of reassembled viewfields of 6x6 x 3 images = 108 images in each panel. Kairos Instruments