immunohistochemistry

Antibody Pair for Protein Protein Interaction and Protein Phosphorylation ( ) - A powerful platform for protein-protein interaction and protein phosphorylation studies in situ is created by combining Abnovas dual recognition, primary antibody pair with proximity ligation assay from Olink Bioscience. This revolutionary combination enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications. More videos at Abnova http

Part 2: SOX2 ***ysis in NSCLC In this talk, a study on SOX2 amplification in non-small cell lung cancer with a special focus on IHC assessment using the Definiens Tissue Studio image ***ysis software will be presented. The aim of the study was to verify SOX2 amplification and protein over-expression in non-small cell lung cancers (NSCLC) and to compare these results with patient and tumor characteristics. A total of 940 NSCLCs from two independent population-based cohorts containing predominantly adenocarcinomas of the lung and squamous cell lung carcinomas, were assessed by fluorescence in-situ hybridization (FISH) and immunohistochemistry (IHC) to study SOX2 amplification and expression level. For protein expression ***ysis, the expression level was explored by utilizing Definiens Tissue Studio.

Proximity Ligation Assay (PLA™) ( ) - Proximity Ligation Assay is a technology that extends the capabilities of traditional immunofluorescence and immunohistochemistry to include direct detection of proteins, protein interactions and modifications with unparalleled specificity and sensitivity. Target proteins are detected with single molecule resolution, allowing digital quantification and information about the exact localization of the target. More videos at Abnova http

Frozen Tissue Array Construction Using Arraymold This demo a simple and easy way to build frozen tissue microarrays using Arraymold. For more information, please visit

How to Interpret Vimentin Immunostain Nearly every day, I see vimentin immunohistochemical stain ordered on spindle cell (and other) tumors. This video explains the proper use and interpretation of vimentin.

Part 2 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project

Skin Cancer Cutaneous T-cell Lymphoma Hematology and pathology Dr. Shane Chapman, MD discusses Skin Cancer Cutaneous T-cell Lymphoma Hematology and pathology. See more at PLEASE RATE AND COMMENT!!! Mycosis fungoides and the Sezary syndrome are cutaneous T-cell lymphomas. These are characterized by the expansion of a clone of malignant CD4 helper T-cells that have a propensity to home and accumulate in the skin, lymph nodes, and peripheral blood. Confirming the diagnosis of mycosis fungoides is difficult in the early stages of the disease. The clinician will biopsy patch stage lesions and ask the pathologist to rule out MF. The classic features of the disease are usually absent at this stage. Typically a few atypical lymphocytes are seen and the pathologist reports an atypical lymphocytic infiltrate, suspicious for MF. Biopsy in patch-or-plaque stage shows a lymphocytic infiltrate in the superficial dermis, with individual lymphocytes migrating among epidermal keratinocytes. The affinity of the malignant T-cells to migrate to the epidermis is called epidermotropism. The lymphocytes are pleomorphic, hyperchromatic, and have hyperconvoluted nuclei. Staining of skin-biopsy specimens with a panel of lymphocyte markers is used sub classify the clone of cells. Immunohistochemistry and molecular genetics have demonstrated aberrant expression of the T lymphocyte surface antigens. The neoplastic T helper CD4 positive cells show gene rearrangements of the T cell receptors, usually of the alpha receptors. Biopsys taken at ...

Lecture 8 Anti-PCNA Antibody Optimization for Histology & Immunohistochemistry Procedures Lecture #8 at Rockefeller University: HCS student, Shelley Jain, presents her PowerPoint on Anti-PCNA Antibody Optimization for Histology & Immunohistochemistry Procedures. Harlem Children Society (HCS: www.hcs2) a Not-for-profit organization founded on June 5, 2000, by Dr. Sat Bhattacharya, selects promising students from communities ridden by poverty and adverse situations, engaging and training them in one-on-one mentoring and hands-on research in STEM and allied sciences. The US HCS Class of 2010 consists of 300 young scholars from over 200 schools from diverse ethnicities (40% African American, 26% Hispanic, 16% Native American, 14% Asian, and 4% Caucasian). They are mentored by over 1500 mentors -- all leading scientists, engineers, doctors and other professionals from over 250 institutions. All HCS students graduating high school students were admitted to college/university, with 20% attending Ivy League schools. "HCS Global Science Parades and Science Street Fairs & Festivals", organized in the Fall each year are the jewels in the crown of the HCS program, culminating in a series of simultaneous worldwide, global webcasted events, where students present their hands-on research projects. The event celebrates the unifying impact of academic inquiry and the human spirit across continental and cultural divides.

Ductal carcinoma in situ (DCIS) simulation -- extended results Agent-based simulation of ductal carcinoma in situ (DCIS), a type of breast cancer that is constrained to growth in the breast duct lumen by a basement membrane. Shown here: a simulation of 45 days' growth in a 1.5 mm length of duct. The mechanical and population dynamic parameters have been calibrated to patient-specific immunohistochemistry and other histologic measurements. Dark circles: cell nuclei Green cells: proliferating cells (Ki-67 positive; S, G2, M, or G1) Red cells: apoptosis cells (cleaved Caspase-3 positive) Pale blue cells: quiescent cells (G0) Dark grey cells: necrotic cells prior to lysis Debris in centre of duct: necrotic cellular debris Red dots in centre of duct: clinically-detectable microcalcifications Method: Agent-based, lattice-free model. Cell velocities determined by balance of adhesive and repulsive forces. Each cell has a phenotypic state governed by stochastic processes derived from nonhomogeneous Poisson processes. Source: Macklin et al. 2011 in review. See: Note: This is an update of the earlier animation at

Georgette Kanmogne, Ph.D., MPH. Georgette Kanmogne, Ph.D., MPH., associate professor, Department of Pharmacology and Experimental Neuroscience. Investigating the mechanisms of HIV-induced vascular dysfunction and neuroAIDS is the focus of Dr. Kanmogne's laboratory. Another focus of her laboratory is nanomedicine and nanotoxicology: study of the ability of antiretroviral drugs-encapsulated nanoparticles to enter the brain, as well as their effects on cell viability and function. For these studies, the laboratory uses various techniques, including molecular biology, gene sequencing and phylogenetic ***yses, gene array and protein microarray, protein chemistry, immunohistochemistry and immunocytochemistry. Video produced by Brandon Poe, AstroPictures Independent Film & Video Productions Music courtesy of ERH

Mobbs ICC Protocol Demonstration of immunohistochemistry protocol.

fly93to09 Dissection of the Adult Drosophila CNS. This is a start-to-finish video showing the dissection of a fruit fly brain. The video begins by introducing the tools and materials and then goes through the process step by step. The method is suitable for downstream staining (eg immunohistochemistry or enzymatic) and is intended to be quick. Because the dissection is so quick, it can be (and is) performed in paraformaldehyde fixative - fixing two birds with one stone as it were. There is always a risk of distortion to the brain during dissection (by stretching) which is somewhat minimised by fixation. Most of the materials should be available in a basic laboratory - the most critical is very very sharp forceps (tweezers). The video shows a sharpening stone and you should sharpen your own tools for the best results. The video has been converted from an old 1993 VHS format with no soundtrack but a written protocol and associated example images have been on-line since the 90's at which I believe makes it one of the earliest on-line protocols in bioscience. Comments welcomed even though I have moved up the evolutionary scale to work on arabidopsis now :) . .

Microtome by Evon Evon shows how to operate a microtome in preparation for immunohistochemical staining and what purpose it serves in the medical field.

Ductal carcinoma in situ (DCIS) simulation Note: An updated version of this is available at: Agent-based simulation of ductal carcinoma in situ (DCIS), a type of breast cancer that is constrained to growth in the breast duct lumen by a basement membrane. Shown here: a simulation of 30 days' growth in a 1mm length of duct. The mechanical and population dynamic parameters have been calibrated to patient-specific immunohistochemistry and other histologic measurements. Dark circles: cell nuclei Green cells: proliferating cells (Ki-67 positive; S, G2, M, or G1) Red cells: apoptosis cells (cleaved Caspase-3 positive) Pale blue cells: quiescent cells (G0) Dark grey cells: necrotic cells prior to lysis Debris in centre of duct: necrotic cellular debris Red dots in centre of duct: clinically-detectable microcalcifications Method: Agent-based, lattice-free model. Cell velocities determined by balance of adhesive and repulsive forces. Each cell has a phenotypic state governed by stochastic processes derived from nonhomogeneous Poisson processes. Source: Macklin et al. 2011 in review. See:

XMRV talk by a TRUE retrovirologist (2 of 3) September 2010: Prostate Cancer & XMRV

CERTL Symposium 2008 - Bhardwaj "Localization of PMNs During Prostate Cancer Progression Detected by Immunohistochemistry" by Anvita Bhardwaj, Mentored by Dr. Purnima Dubey, Pathology, WFUSM

MiniCore Semi-Automated Tissue Arrayer The 3rd generation of MiniCore® reveals itself even simpler to use, more functional with an increased traceability. Discover now the new MiniCore® 3

Tissue Microarray ( ) - Tissue Microarray is a powerful new technology for high throughput ***ysis of protein expression in a large number of tissue samples. Hundreds of tissue cores are arranged on a single slide, and then ***yzed by immunohistochemistry staining. We offer a large collection of immmunohistochmistry (IHC) validated antibodies for the tissue microarray platform. More videos at Abnova http

Lecture 1 Ramanjot - Immunohistochemistry part 2 Lecture #1 at Rockefeller University: Ramanjot Kaur presents her PowerPoint on Immunohistochemistry.Harlem Children Society (HCS: www.hcs2) a Not-for-profit organization founded on June 5, 2000, by Dr. Sat Bhattacharya, selects promising students from communities ridden by poverty and adverse situations, engaging and training them in one-on-one mentoring and hands-on research in STEM and allied sciences. The US HCS Class of 2010 consists of 300 young scholars from over 200 schools from diverse ethnicities (40% African American, 26% Hispanic, 16% Native American, 14% Asian, and 4% Caucasian). They are mentored by over 1500 mentors -- all leading scientists, engineers, doctors and other professionals from over 250 institutions. All HCS students graduating high school students were admitted to college/university, with 20% attending Ivy League schools. "HCS Global Science Parades and Science Street Fairs & Festivals", organized in the Fall each year are the jewels in the crown of the HCS program, culminating in a series of simultaneous worldwide, global webcasted events, where students present their hands-on research projects. The event celebrates the unifying impact of academic inquiry and the human spirit across continental and cultural divides.

Cryostat Tutorial A tutorial for using the cryostat in Histology lab. Students of the UMass Amherst Histology class produced the video.

Part 4 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project

Lecture 1 Ramanjot - Immunohistochemistry Lecture #1 at Rockefeller University: Ramanjot Kaur presents her PowerPoint on Immunohistochemistry.Harlem Children Society (HCS: www.hcs2) a Not-for-profit organization founded on June 5, 2000, by Dr. Sat Bhattacharya, selects promising students from communities ridden by poverty and adverse situations, engaging and training them in one-on-one mentoring and hands-on research in STEM and allied sciences. The US HCS Class of 2010 consists of 300 young scholars from over 200 schools from diverse ethnicities (40% African American, 26% Hispanic, 16% Native American, 14% Asian, and 4% Caucasian). They are mentored by over 1500 mentors -- all leading scientists, engineers, doctors and other professionals from over 250 institutions. All HCS students graduating high school students were admitted to college/university, with 20% attending Ivy League schools. "HCS Global Science Parades and Science Street Fairs & Festivals", organized in the Fall each year are the jewels in the crown of the HCS program, culminating in a series of simultaneous worldwide, global webcasted events, where students present their hands-on research projects. The event celebrates the unifying impact of academic inquiry and the human spirit across continental and cultural divides.

Paraffin Tissue Array Construction Using Arraymold This demo a simple and easy way to build paraffin tissue microarrays using Arraymold. For more information, please visit

Immunohistochemistry ( ) - Immunohistochemistry is a method of detecting the presence of specific proteins in cells or tissues. It is widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue. More videos at Abnova http

VF-300 maintenance This video shows the simple maintenance procedures for the Precisionary Instruments VF-300 Compresstome tissue slicer. This slicer is designed to work for electrophysiology, immunohistochemistry, and confocal microscopy.

Immunihistochemistry on Paraffin Sections of Mouse Epidermis Using Fluoresent Antibodies part 1 Abstract: In the epidermis, immunohistochemistry is an efficient means of localizing specific proteins to their relative expression compartment; namely the basal, suprabasal, and stratum corneum layers. The precise localization within the epidermis of a particular protein lends clues toward its functional role within the epidermis. In this chapter, we describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory. Paraffin sections generally provide much better morphology, hence, superior results and photographs; however, not all antibodies will work with the harsh fixation and treatment involved in their processing. Therefore, the protocol for frozen sectioning is also included. Within paraffin sectioning, two fixation protocols are described (Bouin s and paraformaldehyde); the choice of fixative will be directly related to the antibody specifications and may require another fixing method.

Immunostaining Tutorial In this tutorial we cover the basics of immunostaining mammalian cells with primary and secondary antibodies.

Part 5 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project

Part 3 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project

VF-300 embedding & slicing This video shows how to embed the tissue and slice tissues for the Precisionary Instruments VF-300 Compresstome tissue slicer. This slicer is designed to work for electrophysiology, immunohistochemistry, and confocal microscopy.

tissue embedding and slicing -- Precisionary Instruments VF-700 Compresstome This video describes how tissue embedding and slicing in the Precisionary Instruments VF-700 Compresstome works.

Part 1 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project

Immunihistochemistry on Paraffin Sections of Mouse Epidermis Using Fluoresent Antibodies part 2 Abstract: In the epidermis, immunohistochemistry is an efficient means of localizing specific proteins to their relative expression compartment; namely the basal, suprabasal, and stratum corneum layers. The precise localization within the epidermis of a particular protein lends clues toward its functional role within the epidermis. In this chapter, we describe a reliable method for immunolocalization within the epidermis modified for both frozen and paraffin sections that we use very routinely in our laboratory. Paraffin sections generally provide much better morphology, hence, superior results and photographs; however, not all antibodies will work with the harsh fixation and treatment involved in their processing. Therefore, the protocol for frozen sectioning is also included. Within paraffin sectioning, two fixation protocols are described (Bouin s and paraformaldehyde); the choice of fixative will be directly related to the antibody specifications and may require another fixing method.

Immunohistochemistry staining steps Immunohistochemistry staining steps. Visit my web site : mimoza.marmara.edu.tr

Immunofluorescence This video explains how to use immunofluorescence to stain different parts of the cell.

Antibody Conjugation - FITC ( ) - The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophore's high quantum efficiency and conjugate stability. More videos at Abnova http

Digital Pathology - What does going digital look like to a pathologist? (English) Product video of Aperio's Digital Pathology Solution for Immunohistochemistry (IHC) featuring the digital workflow of a pathologist. What does the promise of the digital revolution in medicine hold for pathology - a more efficient work flow? New and better tools? Take a look and judge yourself. Find out more at: /IHC.

VF-300 changing & aligning blades This video shows how to change blades and align the blade for tissue slicing for the Precisionary Instruments VF-300 Compresstome tissue slicer. This slicer is designed to work for electrophysiology, immunohistochemistry, and confocal microscopy.

Quick-Ray Manual Tissue Microarrayer Quick-Ray Manual Tissue Arrayer is a device manufactured with the latest state-of-the art technology , allows the researcher to assemble tissue specimens of multiple patients on one slide. It is not only decreases the use of reagent, time and human resource, but also can be applied to almost any tissue processing and ***yzing needed for immunohistochemistry, in situ hybridization, FISH and in situ PCR.

VF-300 components The different components and ingredients needed to use the Precisionary Instruments VF-300 Compresstome tissue slicer. This slicer is designed to work for electrophysiology, immunohistochemistry, and confocal microscopy.